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How To Interpret Hplc Data. As you noted in the introductory lab sesssion the HPLC system gives out two types of data images. When you have done this you should submit your sample using the procedure shown in the video below. Coupling HPLC to a mass spectrometer. Launch the HPLC data processing software by clicking once on the icon.
With NH4 if. One is called a chromatogram and the other is called a spectrum. Popular Answers 1 First locate the signal for the protonated molecule if present. As you noted in the introductory lab sesssion the HPLC system gives out two types of data images. You will use a copy of the HPLC software that is not attached to the instrument to. Alteration in temperature and presence of dust in chromatography lab can greatly vary the result output.
When you arrive for your HPLC session start by preparing the sample using the procedure in the lab manual.
Liquid chromatography - mass spectrometry LC-MS combines two approaches to separate and identify molecules or compounds present in a sample. Launch HPLC Data Processing Software You should see one icon in the Applications box. If the HPLC Data Processing icon is not present ask your TA for help. When you have done this you should submit your sample using the procedure shown in the video below. After the method development is complete a typical workflow for sample analysis is as followsi Measurement of the peak areas of various known concentration of the calibration standardii Plot. It is not necessarily the peak at the highest mz value as you could have formed adducts eg.