02
Jun
Estimation Of Amylase Activity By Dns Method. Design-response surface methodology by dinitrosalicylic acid method Abstract The optimum conditions of an α-amylase activity were experimentally investigated in details comparing with those of using a response surface methodology RSM. Dilute to a final volume of 100 ml with reagent grade water. Dissolve 16 gm of NaOH in 20ml of distilled water. The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product nitro-amino-salicylic acid.
DNS reagent 35-dihydrosalicylicacid in 04 M NaOH spectrometrically at 540 nm. 2 N Sodium hydroxide. 23 Inhibition assay of amylase inhibitor on αααα-amylases Alpha-amylase inhibitory activity was measured by adding equal. Take the tubes and cool to room temperature. This was the original equation. 3 5-Dinitrosalicylic acid DNSA is used extensively in biochemistry for the estimation of reducing sugars.
Add 20 ml of 2 N NaOH.
It detects the presence of free. Starch H2O α-Amylase Maltose reducing agent. Dissolve 16 gm of NaOH in 20ml of distilled water. Dinitrosalicylic acid color reagent. DNS method The DNS method for estimating the concentration of reducing sugars in a sample Reducing sugars contain free carbonyl group have the property to reduce many of the reagents. 05 ml of properly diluted in acetic acid buffer solution.