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Dna Directed Rna Polymerase. Forms the polymerase active center together with the second largest subunit. Can initiate a chain de novo. Wenn sich an eine Base der DNA ein unpassendes RNA- Nukleotid anlagert so verbleibt die RNA-Polymerase länger an der entsprechenden DNA-Stelle. In Arabidopsis DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 DRM2 and targeted by 24-nucleotide small interfering RNAs siRNAs through a pathway termed RNA-directed DNA methylation RdDM.
Nucleic Acids Res 21 49044908 1993 PubMed CrossRef Google Scholar. Catalyses DNA-template-directed extension of the 3- end of an RNA strand by one nucleotide at a time. Characterisation of the enzyme and the DNA-sequence of the genes for the large subunits. This process differs slightly in eukaryotes and prokaryotes. In Arabidopsis DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 DRM2 and targeted by 24-nucleotide small interfering RNAs siRNAs through a pathway termed RNA-directed DNA methylation RdDM. In any case viroid replication triggers the hosts RNAi mechanism 1820.
Characterisation of the enzyme and the DNA-sequence of the genes for the large subunits.
A single stranded DNA template strand of the promoter is positioned. Whether the RNA-directed RNA polymerase RDR transcription of these oligomeric transcripts or even of the mature viroid contributes to dsRNA generation is not clear but cannot be excluded. DNA-directed RNA polymerase Virally encoded protein EC 2776 which synthesizes RNA using DNA as a template. This pathway requires two plant-specific RNA polymerases. This enzyme is able to synthesize a double helix DNA once the RNA has been reverse transcribed in a first step into a single-strand DNA. This enzyme activity might be necessary for the.