29
Oct
6 Ohda Parkinson Model. Knockdown of FTH1 in PC-12 cells significantly inhibited. 6-OHDA Unilateral Lesion Rat Model of Parkinsons Disease Parkinsons disease PD is a neurodegenerative disorder that affects about 15 of the global population over 65 years of age. When injected stereotaxically into the brain either into the median forebrain bundle MFB or into the neostriatum it causes extensive. To develop the 6-OHDA model neurotoxin has to be injected into distinct parts of the animal nigrostriatal pathway.
When injected into the striatum of rats 6-OHDA produces a protracted retrograde degeneration of nigro-striatal neurons over several weeks and leads to a stable and permanent depletion of tyrosine hydroxylase TH-positive nigral neurons Sauer and Oertel 1994. This study was undertaken to examine the beneficial effects of GEB on L-DOPA induced dyskinesia in 6-hydroxydopamine 6-OHDA-induced experimental Parkinsonism. Med vorgelegt der Medizinischen Fakultät Charité Universitätsmedizin Berlin von Johanna Kühn aus Gengenbach Datum der Promotion. To develop the 6-OHDA model neurotoxin has to be injected into distinct parts of the animal nigrostriatal pathway. Substantia nigra pars compacta. The neurotoxin 6-hydroxydopamine 6-OHDA is widely used to induce depletion of dopaminergic neurons in animal models of Parkinsons Disease PD.
The 6-hydroxydopamine 6-OHDA model of Parkinsons disease PD is one of the most extensively utilized animal models used to study pathogenic processes involved in neuronal loss and behavioral alterations characteristic for parkinsonism.
6-OHDA is a highly specific neurotoxin which targets catecholamine neurones via the dopamine active transporter DAT. The most frequently used toxins in rodent models of PD is either the neurotoxin 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine MPTP or 6-hydroxydopamine 6-OHDA. We now know that the model induced by 6-OHDA does not include all PD symptoms although it does reproduce the main cellular processes involved in PD such as oxidative stress neurodegeneration neuroinflammation and neuronal death by apoptosis. When injected stereotaxically into the brain either into the median forebrain bundle MFB or into the neostriatum it causes extensive. When injected into the striatum of rats 6-OHDA produces a protracted retrograde degeneration of nigro-striatal neurons over several weeks and leads to a stable and permanent depletion of tyrosine hydroxylase TH-positive nigral neurons Sauer and Oertel 1994. Our present work demonstrates that FTH1 is involved in iron accumulation and the ferroptosis pathway in this model.